Localization of kallikrein in salivary glands of the Japanese monkey, <I>Macaca fuscata</I>

概要

論文の詳細を見る
Although Kallikrein has previously been localized in the duct cells of salivary glands, the manner in which it enters the saliva remains unclear. We used both immunofluorescein and protein A-gold methods at the light microscopic level and the protein A-gold method at the electron microscopic level to determine the distribution of kallikrein antigenicity in monkey salivary glands. The specificity of anti-human urinarykallikrein to glandular kallikrein in the monkey submandibular gland was demonstrated by Western blotting. Light microscopic immunohistochemical staining was intense at the luminal rim of the striated duct cells, but was less intense in the excretory duct cells, and was faint in the intercalated duct cells, Among the major salivary glands, staining intensity paralleled the degree of development of the striated ducts, with the submandibular gland exhibiting the most intense staining, the parotid to a somewhat lesser degree, and the sublingual displaying only minor staining. In the apical cytoplasm of the submandibular striated duct cells, some small granules and vesicles and vacuoles were observed near the luminal plasma membrane. By ultrastructural immunohistochemical staining, kallikrein appeared to be localized in small secretory granules. Because some of the small granules seemed to be attached to the luminal membrane, we suggest that kallikrein is secreted by exocytosis from the duct cells.
歯科基礎医学会の論文