A Study of the Apatite-Collagen Composite. Part III. Solubility of collagen and cytotoxicity of the composite.:Part III: Solubility of collagen and cytotoxicity of the composite

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A mixture of non-sintered CO3apatite and enzymatically-treated collagenwas produced and named as an apatite-collagen composite. UV ray-irradiation had been applied to increase insolubility of CO3apatite-collagen composite by enhancing cross-links of the collagen fibers. The effect of UV ray-irradiation on the atelocollagen, one main component of CO3apatite-collagencomposite, was investigated. Solubility of collagen gel was analyzed by hydroxyproline assayafter collagenasedigestion. Insolubility of the composite-collagen after UV ray-irradiation was also studied. The results showed that UV ray-irradiation on the apatite-collagen composite is a reliable method to increase insolubility of the composite. To investigate the biocompatibility of the composite in vitro, cytotoxicity test by L-929 cell culture was carried out. Cell growth in UVray-irradiated composite-dissolved MEM was no less than the control MEM, and thecomposite could be regarded as biocompatible.
歯科基礎医学会の論文