Effect of testosterone propionate and/or insulin on the osteoblastic cell line: MC3T3-E1.
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Effects of testosterone propionate (TP) and/or insulin (Ins) on the proliferation and differentiation in the osteoblastic cell line: MC3T3-E1 were studied. MC3T3-E1 cells were incubated in a serum-free α-MEM medium containing 0.1% BSA with or without 10<SUP>-9</SUP>-10<SUP>-6</SUP>M TP and/or 10<SUP>-9</SUP>-10<SUP>-6</SUP>M Ins, and[<SUP>3</SUP>H]thymidine incorporation into the cell was assayed for assessment of DNA synthesis, and the ALPase activity of the cell was also assayed. Results were obtained as follow: 1. The time related decrease of DNA synthesis of MC3T3-E1 cells in serum-free medium was strongly inhibited by Ins. TP showed weaker effect than Ins. Combination of Ins and TP showed a weak synergistic effect. 2. TP significantly inhibited ALPase activity in dose-dependent manner. Ins also showed a tendency to inhibit ALPase activity. And the effects were strengthened by the combination of both, hormones. The effects of the Ins and TP on MC3T3-E1 cells will be expressed through the acceleration of proliferation ability of the cell, and the effect of Ins is stronger than TP.
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