Characterization of arsenate-, selenate- and nitrate-reducing activities in Bacillus sp. SF-1

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A gram-positive and facultative anaerobic bacterium, <I>Bacillus</I> sp. SF-1, which was isolated from selenium-polluted sediment, can grow on lactate as an electron donor and selenate, arsenate or nitrate as an alternate electron acceptor in the absence of oxygen. This strain seems to be a promising agent for bioremediation of selenium/arsenic. In an effort to characterize the reductase systems in strain SF-1, arsenate, selenate and nitrate reduction activities were investigated with washed-cell suspensions and crude cell extracts from cells grown on arsenate, selenate and nitrate. These reductase activities were induced individually by the respective electron acceptors. Tungstate, which is a typical inhibitory antagonist of molybdenum-containing dissimilatory reductases, strongly inhibited reduction of arsenate, selenate and nitrate in the anaerobic growth cultures. These results suggested that strain SF-1 catalyzes the reduction of arsenate, selenate and nitrate by distinct terminal reductases containing molybdenum as a cofactor. This may be an advantage to utilize SF-1 in the bioremediation precesses where contaminants exist in mixture.
日本水処理生物学会の論文