Behavior analysis by the antigen-antibody method of nitrifying bacteria immobilized on PEG carrier by inclusion or adhesion method.
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概要
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Monoclonal antibody method is very useful and helpful for <I>in situ</I> detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, the specificities of monoclonal antibodies raised against <I>Nitrosomonas europaea</I> (IFO14298) and <I>Nitrobacter winogradskyi</I> (IFO14297) were checked by sandwich ELISA. As a result, it was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria and specificity for various genus and species of nitrifying bacteria. The spatial distribution of <I>N. europaea</I> and <I>N. winogradskyi</I> immobilized by entrapped-type and attached-type PEG gel pellet introduced into the nitrification reactor was examined by<I> in situ</I> fluorescent antibody technique. As a result, it was found that <I>N. winogradskyi</I> was more sensitive to the damage during entrapping process than <I>N. europaea.</I> Whereas <I>N. winogradskyi</I> existed only near the surface area, <I>N. europaea</I> existed near the surface area and at inner part of gel pellet. On the other hand, in attached-type PEG gel pellet, the high density areas of <I>N. winogradskyi</I> were observed compared with entrapped-type PEG gel pellet. It was suggested that this fluorescent antibody technique was utilized for understanding the microbial community of nitrifying bacteria as a quick and favorable tool.
- 日本水処理生物学会の論文
日本水処理生物学会 | 論文
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