Identification of Bacteria in Activated Sludge. A Simple Testing Method Comparison.

概要

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Some simple testing methods for identification of bacteria in activated sludge by using 511 bacterial isolates were evaluated, and the following results were obtained.<BR>(1) Compared with the Gram staining method, the caustic potash method (Ryu's method) was equally reliable, easier and less troublesome.<BR>(2) Three kinds of motility test methods were compared; (i) cultivation with semi-solid medium, (ii) microscopic examination and (iii) staining of bacterial flagella. The agreement of the test results between (i) and (ii), and between (ii) and (iii) were 89.8% and 69.6%, respectively. The microscopic examination method was the easiest and most reliable.<BR>(3) Three kinds of cytochrome oxidase reagents from different suppliers were tested and compared: one was the dropper-type reagent which seals Kovac's reagent in an ampule and is used by dropping it on a filter paper streaked with bacteria. The other two were filter paper-type reagents which contain Kovac's reagent in a filter paper and are used by rubbing bacteria with a platinum loop. The agreement of the results by three test reagents was 85.4%.
日本水処理生物学会の論文