Growth-Inhibition of Baker's Yeast by Cationic Surface-Active Agents (III):Elution of Detergents from the Yeast by Washing and Reversal of Its Growth-Inhibition

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When yeast cells suspended for ten minutes in a solution containing each of four kinds of cationic detergents at a concentration of 0.25mM respectively were washed 3 times with water, 11% to 83% of the adsorbed detergents were removed. However, no significant reversal of the growth-inhibition was observed after such washing, as seen in Fig. 1. On the other hand, effects on the cell of washing with 10<SUP>-4</SUP>M anionic detergents such as sodium lauryl sulfate or sodium laurate were presented in Fig. 3. Cells treated with cetyldimethylbenzylammonium chloride (C-Bz) or lauryl-3-chloro-2-hydroxypropylammonium chloride (L-Ep) showed considerable reversal of the growth-inhibition, while the growth of the cells treated with lauryldimethylbenzylammonium chloride (L-Bz) was more strongly inhibited by repeated washing with anionic detergents. As shown in Fig. 4, among the cationic detergents, C-Bz was the most efficient both in the degree and the rapidity of growth-inhibition, and the effectiveness decreased in the order of L-Ep, L-Bz, and cetyl-3-chloro-2-hydroxypropylammonium chloride (C-Ep). However, a relation between the effectiveness of a cationic detergent and the reversal of growth-inhibition by its elution was not clear.
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