Development of the Mouse Passive Abdominal Wall Anaphylaxis (PAA) Method and Application of the Method to Search for Anti-allergic Effect of Foods

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Food allergy, which generally belongs to the category of Type I hypersensitivity reaction, is a serious health problem. Previously, we reported two types of mouse anaphylactic models, one using the abdominal wall as a site for both induction and estimation (AW method), and the other estimating the hypotension in mice sensitized passively with mouse monoclonal anti-DNP IgE.In this paper, we propose a passive abdominal wall anaphylaxis (PAA) method for use in screening for anti-allergic substances. Normal mice were intraperitoneally sensitized with mouse monoclonal anti-DNP IgE (5μg/mouse) and 24 hours later, challenged with dinitrophenyl human serum albumin (10μg/50μL/site) on the abdominal wall. The PAA was estimated in terms of the increase of vascular permeability at the challenged abdominal wall site.The PAA was inhibited by the oral preadministration of diphenhydramine in a dosedependent manner (PAA inhibition, PAAI). The anti-allergic activities of such well-known anti-allergic foods as oolong tea, garlic, and leek were confirmed by the PAAI test. Therefore, the PAA method appears to be applicable for the screening of anti-allergic substances in foods, simply, rapidly, and with high sensitivity.
公益社団法人日本食品衛生学会の論文