Dissociation of azo dye-treated heterochromatin and release of non-histone proteins.:Paper No. 10 of the series "The Effect of Tar Dyes on RNA Synthesis"

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Amaranth (FD & Red C No. 2) and Ponceau 3R stimulate <I>in vitro</I> RNA synthesis by causing the dissociation of chromatin in isolated rat-liver nuclei. To elucidate the mechanism of chromatin dissociation, the effect of amaranth on chromosomal proteins was examined using heterochromatin prepared from isolated rat-liver nuclei. The dissociation of amaranth-or Ponceau 3R-treated heterochromatin was confirmed by both solubility measurement and electron microscopy. Amaranth released nonspecific non-histone proteins and increase in its concentration resulted in the release of non-histone proteins with molecular weights of 17.5, 25, and 31 kilodaltons in addition to the nonspecific non-histone proteins. These non-histone proteins were presumed to be high mobility group (HMG) proteins from their behavior on SDS-electrophoresis. On the other hand, no release of histone was observed in these experiments.
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