A New Simplified Method for Microdetermination of Mercury in Fish by Oxygen-Flask Combustion Method and by Flameless Atomic Absorption Spectrophotometry

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A new simplified method for microdetermination of mercury in fish is described. In this method, the two necked oxygen-flask with two necks of 1000ml capacity was used for burning the biological material. Before the sample was burnt in the oxygen-flask, the oxygen-flask was evacuated with a vacuum pump, then the oxygen-flask was filled with oxygen. These operations were repeated three times, and at the end of these operations the internal pressure of the oxygen-flask was adjusted to about 610mm Hg for avoidance of explosion of the oxygen flask and loss of mercury. After the sample was burnt, the oxygen-flask was cooled with a dry ice-acetone mixture to condense the vaporized mercury. Then the mercury and some vaporized components were absorbed to 0.1N sulfuric acid. The volatile components except mercury in the absorbant solution were extracted with n-hexane, the water layer being used as the prepared solution. The mercury in the prepared solution was determined by flameless atomic absorption spectrometry. As a result of repeating this method on a few kinds of fish, it was found that the mercury in fish sample could be determined with 100-105% of recovery ratio and about 5% of variation coefficient.
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