A Study of Lipid Biosynthesis in Cryptococcus neoformans.
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概要
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Changes in incorporation of [14C] acetate into lipid fractions were examined in Cryptococcus neoformans during growth and were compared with those in Candida albicans. The uptake of [14C] acetate in the total lipids of Cr. neoformans gradually increased following the growth phase and then decreased at the stationary phase. The hydrolysis of triacylglycerol (TG), the main lipid in Cr. neoformans, was considered to be accelerated as the radioactivity in TG decreased with a concomitant increase of radioactivity in free fatty acid (FFA) in the stationary phase. In contrast, in C. albicans, phosphatidylcholine (PC), the major component of cell membrane, increased during the growth phase and decreased in the stationary phase. It was also observed that the biosynthesis of acidic phospholipids, PI and PS, was enhanced during the growth phase in both types of fungi.
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