Elastase Activity from Aspergillus Species and Inhibition by Urinastatin.
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概要
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Fourteen strains of Aspergillus fumigatus, two strains of A. flavus, three strains of A. niger, and one strain of A. candidus were isolated from sputa of patients with respiratory diseases. These Aspergillus strains were incubated for 7 days at 37°C in 0.05% yeast carbon base (YCB) agar plates containing 0.1% rose bengal and 0.05% elastin. Elastase activity of Aspergillus species, and the effect of Urinastatin on this activity were examined. Five strains of A. fumigatus, one of A. flaves, and one of A. niger showed the activity.Elastase activity of these strains was inhibited by Urinastatin. It is suggested that the combination therapy of an enzyme inhibitor, such as Urinastatin, and an anti-fungal agent is more effective for aspergillosis than the agent alone.
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