Polymerase Chain Reaction for the Diagnosis of Candidiasis: Detection of Candida albicans DNA in Cerebrospinal Fluid.
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概要
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A method utilizing the polymerase chain reaction (PCR) for amplification of<I>Candida albicans</I>DNA has been developed for diagnosis of candidiasis. The specific oligonucleotide primers used were selected on the basis of the nucleotide sequence of a gene encoding the secretory aspartate proteinase of<I>C. albicans</I>.<BR>A 10-year-old girl who had symptoms compatible with meningitis due to fungal infection was studied by PCR. A 273-base-pair DNA fragment from cerebrospinal fluid (CSF) was amplified and the specificity was confirmed by Southern blotting analysis using a specific oligonucleotide probe. Although there was no obvious etiologic route of fungal infection of the CSF in this patient, cultures taken from the CSF were positive for<I>C. albicans</I>.<BR>It has been difficult to diagnose Candida meningitis early, because cultures from CSF are often negative for<I>Candida</I>. This study demonstrated that PCR can be useful as a rapid and sensitive diagnostic method for cases of fungal infection, as has already been shown for viral and bacterial infection.
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