Biochemical and Immunological Studies of Neutral Protease. Localization and Extraction in vivo.:Localization and Extraction in vivo
スポンサーリンク
概要
- 論文の詳細を見る
Yu, et al and Day, et al reported of the isolation and purification of keratinase from Trichophyton mentagrophytes in 1968. Release of proteolytic enzyme from the dermatophytes is assumed to be essential for their persistence, and thus has been the subject of many investigations. We also have reported isolation and purification of extracellular keratinase (e-kase) from Microsporum gypseum and Microsporum canis. In this report, we summarize our previous studies and, in addition, report new data on the quantification of e-kase extracted from lesional skin.1. E-kase produced by M. canis was serine protease. The enzyme was induced by the addition of human hair instead of glucose and pepton in the culture medium. Its molecular weight is 32, 500 determined by SDS PAGE with a pH optimun of 7.48.2. We produced both polyclonal and monoclonal anti-e-kase IgG. Four proteases from M. canis, M. gypseum, T. mentagrophytes, Trichophyton rubrum were produced and purified. Immunological identity between these four e-kase was confirmed by immunodiffusion and western blot analysis.3. Localization of e-kase in clinical lesion was examined immunoenzymatically with light and electron microscopy. The results were as follows: 1) protease reactions surrounded fungal elements in the horny layer, 2) digestion of both tonofilament and marginal band of horny cells was also observed.4. Protease was extracted and detected with EIA method from the lesional skin. Protease detected was 346ng/g of horny layer cells. This is the first successful report of quantification of e-kase in skin lesion.
- 日本医真菌学会の論文
日本医真菌学会 | 論文
- (1→3)-β-D-グルカン測定の問題点と進歩
- 両側の肺空洞病変にアスペルギルス感染を合併し抗真菌薬, ミカファンギンとイトラコナゾールの併用療法が有効であった Wegener 肉芽腫症の一例
- Trichophyton tonsurans 臨床分離株に対する各種抗真菌薬の in vitro 抗真菌活性
- 爪白癬の治療について : 日本医真菌学会標準化委員会提案2007
- Candida 属菌種のアゾール系薬感受性試験における現行の日本医真菌学会法, およびその改変法とNCCLS M27-A2法との相関性に関する検討