Effect of SS717 on the ultrastructure of Trichophyton mentagrophytes as observed by scanning and transmission electron microscopy.

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The effect of a new imidazole antimycotic, SS717, on the morphology of growing hyphae of Trichophyton mentagrophytes cultures in a liquid medium was investigated by scanning and transmission electron microscopy. Hyphal growth in broth was inhibited at the drug concentration of 3.1ng/m/. The most prominent morphological change seen in cultures with relatively low concentrations of the drug was swelling of the tip of hyphae. In ultrathin sections, thickening of the cell wall and accumulation of electron dense granular structures formed within both the wall and cytoplasm were also observed. Alterations of the ultrastructure of intracytoplasmic organelles increased in extent with increasing drug concentration and incubation time. After exposure to 310ng/ ml of SS717 for 48 hours, most of the fungal cells became deteriorated into ghosts in which normal intracellular structures and matrix were lost. On the other hand, the cells exposed to a fungicidal dose (1.5-3.1 μg/ml) of SS717 were led to necrosis with degeneration of intracytoplasmic membrane systems within 6 hours.
日本医真菌学会の論文