Experimental tinea pedis in guinea pig feet. Scanning electron microscopic and histological study of the infection.:Scanning Electron Microscopic and Histological Study of the Infection
スポンサーリンク
概要
- 論文の詳細を見る
Arthrospores of Trichophyton mentagrophytes were inoculated onto the plantar part of a guinea pig foot by the wet disc inoculation method.Anthropophilic strain NTM-105 and zoophilic strain SM-110 required 280 arthrospores (or 315 microconidia) and 80 arthrospores to infect 50% of the inoculated feet, respectively. After inoculation of 5×103 arthrospores, all feet were infected, even 50 arthrospores were shown to be infective in some cases.Some arthrospores of NTM-105 inoculated on plantar parts of guinea pig feet germinated in 6 hours and the hyphae invaded horny cells in 12 hours, but histological examination revealed no fungal elements in the horny layer of 24-hour feet. After 3 days, one-third of the horny layer was invaded by fungi and after 7 days this had increased to two-thirds.Anthropophilic strain NTM-105 invaded only the upper two-thirds of the horny layer and induced no inflammatory response. On the other hand, zoophilic strain SM-110 invaded the whole horny layer and provoked strong inflammatory response and clinical manifestations.Infecting fungi were demonstrated in the horny layer of all inoculated feet for more than 6 months.The experimental infection indicated herein will be a useful model to analyse tinea pedis, because it is quite similar to human tinea pedis histologically, clinically and in terms of its chronicity. Precise experimental studies using this model may open the way to an elucidation of fungal infection mechanisms against host defense activities and an evaluation of antifungal treatments.
- 日本医真菌学会の論文
日本医真菌学会 | 論文
- (1→3)-β-D-グルカン測定の問題点と進歩
- 両側の肺空洞病変にアスペルギルス感染を合併し抗真菌薬, ミカファンギンとイトラコナゾールの併用療法が有効であった Wegener 肉芽腫症の一例
- Trichophyton tonsurans 臨床分離株に対する各種抗真菌薬の in vitro 抗真菌活性
- 爪白癬の治療について : 日本医真菌学会標準化委員会提案2007
- Candida 属菌種のアゾール系薬感受性試験における現行の日本医真菌学会法, およびその改変法とNCCLS M27-A2法との相関性に関する検討