Partial purification and the localization of keratinase in plantar horny layer infected with tinea pedis.
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概要
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Keratinase was partially purified from plantar horny layer infected with Trichophyton rubrum by procedures of homogenization, ultrafiltration, gelfiltration and ion exchange chromatography. When enzyme-containing fractions were tested by the addition of anti-keratinase IgG, significant decrease of the activity was detected.The partial or complete disappearance of tonofilaments, cell membranes, including a marginal band of horny cells around fungi was observed by electron microscopy. The presence of keratinase in the horny cell of tinea pedis was confirmed by immuno-electron-microscopy using rabbit anti-keratinase IgG and peroxidase conjugate of goat antirabbit IgG. The immuno-reactive protein was located not only around the fungal cell-wall but also around the horny cell-membrane, including marginal band and intercellular space.
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