Studies on toxin of Aspergillus fumigatus. XVII. Changes of biological activities accompanied by the inactivation of Asp-hemolysin.

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The hemolytic activity of Asp-hemolysin was remarkably inhibited by the addition of chemical reagents such as HgCl2 and iodine. Injection of Asp-hemolysin-Hg2+ by the intravenous route showed strong lethality to mice, but only a poor lethal toxicity by the i. v injection of Asp-hemolysin-Hg2+ and by the i. v and i. p injection of Asp-hemolysin-I2. The hemolytic activity of Asp-hemolysin inhibited by HgCl2 was excellently restored by the addition of each homogenate-extract prepared from the liver and kidney of mice.Egg albumin, polypeptone, and proteose peptone showed little restration of hemolytic activity of Asp-hemolysin-Hg2+ against hen erythrocytes. Under the same conditions, mouse serum had little effect to Asp-hemolysin-I2. On the other hand, the hemolytic activity of Asp-hemolysin inhibited by iodine could not be restored after the addition of homogenate-extract, protein preparation, and serum, respectively. The enzymatic hydrolysis of native Asp-hemolysin was not found by the treatment with several proteolytic enzymes, while Asp-hemolysin-Hg2+ could be hydrolyzed after treatment with proteolytic enzyme such as α-chymotrypsin, trypsin, pronase P, and A. fumigatus protease. In addition, the hemolytic activity of Asp-hemolysin-Hg2+ was not effected by the treatment with trypsin for 150 minutes, but lethal toxicity to mice had yet remained in the toxin. When Asp-hemolysin was treated with thermolysin at 50°C, both activities of hemolysis and lethality were completely inactivated. These results suggest that the hemolytic activity of Asp-hemolysin can not be related to its lethal activity.
日本医真菌学会の論文

Studies on toxin of Aspergillus fumigatus. XVII. Changes of biological activities accompanied by the inactivation of Asp-hemolysin.

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日本医真菌学会 | 論文

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