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We obtained Candida albicans cells grown predominantly in the yeast-like (Y) and mycelial (M) forms, which developed in Sabouraud's glucose medium and in synthetic media containing methionine, respectively. Protoplasts were prepared from the two forms by using a cell wall lytic enzyme (Zymolyase) from Arthrobacter luteus and plasma membranes were isolated by hypotonic disruption of protoplasts and differential centrifugation. The comparative lipid analysis of plasma membranes thus obtained showed that sterol content of the M-form cells was approximately three times greater than that of the Y-form cells. Major polar lipids were phosphatidylcoline and phosphatidylethanolamine, and their fatty acids were mainly palmitic, palmitoleic, oleic and linoleic acids. No marked difference was observed in the phospholipid class composition between two forms, whereas there was a greater proportion of oleic acid in the Y-form cells. Data of ESR spectra obtained by using a spin probe (5 SAL) demonstrated that the order parameter of plasma membrane lipids from the M-form cells was greater than that from the Y-form cells. It implies that the physical state of the M-form plasma membrane is different from that of the Y-form plasma membrane, the former being less fluid. The activity of chitin synthetase (EC: 2.4.1.16) which is associated with plasma membranes was approximately three to five times greater in the M-form than in the Y-form. These results provide evidence which suggests that the lipid composition of plasma membranes would be somehow related with the dimorphic morphogenesis of Candida albicans.
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