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Antigenic analyses of four serotypes of Cryptococcus neoformans were carried out by absorption with reciprocal absorption experiments, and the antigenic patterns of four serotypes were established. The serotypes A, D, B and C demonstrated antigenic factors 1, 2, 3, 7; 1, 2, 3, 8; 1, 2, 4, 5; and 1, 4, 6; respectively. Comparative experiments by our system for serotyping of C. neoformans isolated from Japan indicated that 49 out of 52 strains were identified as serotype A. Remaining 3 strains exhibited antigenic factors 1, 2, 3, 7 and 8. Therefore, we designated them as serotype A-D tentatively. It was suggested that 8 kinds of factor sera were useful for identifying the serotype of C. neoformans isolates, and the majority of isolates in Japan were serotype A.
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