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A partially purified extracellular keratinase obtained from the culture filtrate of a Microsporum gypseum strain showed two separate bands on disc electrophoresis at pH 4.0 gel. Keratinase activity was detected in accordance with the second band on this gel. The keratinase was activated by calcium but inhibited by magnesium, manganese and EDTA. The keratinase was also activated by the presence of thiol reagents such as 2-ME and DTT, whereas its inhibition was observed under the presence of iodoacetamide and NEM. The DFP has no apparent effect on the keratinase activity. The keratinase activity was inhibited by adding whole human serum. This inhibitory effect of the serum was lost after treating with anti-α2-macroglobulin. Immunoelectrophoretic analysis revealed the presence of α2-macroglobulin in the vesicle fluid from a patient with Tinea pedis. These results suggest that the keratinase might be one of thiol proteases and inhibited by human α2-macroglobulin.
- 日本医真菌学会の論文
日本医真菌学会 | 論文
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