New rapid clean-up device for ochratoxin A
スポンサーリンク
概要
- 論文の詳細を見る
High Performance Liquid Chromatography (HPLC) with fluorescence detection (FLD) and Thin Layer Chromatography (TLC) are widely used analytical methods for the determination of Ochratoxin A (OTA). As normally the crude extract cannot be used directly for these analyses, a clean-up step is necessary. Commonly used clean-up procedures comprise immunoaffinity columns (IAC), liquid liquid extraction (LLE) and solid phase extraction (SPE). The performance of a new single step clean-up column Mycosep® 229 Ochra from Romerlabs® was tested in matrices often contaminated with OTA like green coffee beans, raisins and various kinds of cereals. In all these matrices an average recovery of more than 90% (range 2.6-92μg/kg; n=24) of the added OTA could be obtained. Additionally, a certified reference material (CRM), OTA in wheat, was tested with the column. An average recovery of 96.2% (n=9) was achieved for the CRM proving the applicability and high accuracy of the developed method.
- 日本マイコトキシン学会の論文
日本マイコトキシン学会 | 論文
- コメ中のフモニシンB1, B2, B3の液体クロマトグラフィーによる定量
- 発がん物質にも閾値が存在する
- Aspergillus novofumigatus から分離されたジヒドロテレインおよびテレインの血小板凝集作用に関する検討
- マルブランキア・フィラメントサから得られる2種の新規フラノン誘導体
- ブラジル土壌より分離されたアスペルジルス・フミガツスIFM 54246株から得られた抗真菌活性物質