Information on the cell cycle for diagnosis of lymphoma using 7AAD/ PY.
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概要
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We developed an improved technique that permits simultaneous surface phenotyping and DNA/RNA quantitation by a flow cytofluorometry using FITC-conjugated monoclonal antibodies, 7-amino-actinomycin D and pyronin Y, respectively. Clinical samples from patients with non-Hodgkin's lymphoma were analyzed for cell cycle using 7AAD/PY. Pathological lymphoma cells and reactive normal lymphocytes from clinical samples were separately analyzed for ploidy, cell growth and light scattering profiles. The effect of growth factors on pathological cells was also analyzed using 7AAD/PY in vitro. 7AAD/PY may be useful for clinical diagnosis of lymphoma, as well as predicting the effectiveness of chemotherapy and the prognosis of disease in a clinical setting and to analyze in the vitro effects of biological effectors such as chemotherapy drugs, growth factors and other biological modifying factors.
- 日本リンパ網内系学会の論文
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