Studies regarding to the effect of circulating endothelial cells on cerebral embolisms
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It has been known that exfoliation of endothelial cells is a factor of atherogenesis. We observed the circulating exfoliated endothelial cells in man with Giemsa staining in accordance with the method of Hladovec, further with Ruthenium Red staining and direct immunofluorescent staining using anti-human factor VIII serum and using antihuman α<SUB>2</SUB>-macroglobulin serum.<BR>For the purpose of examining our studies that circulating endothelial cells can cause cerebral infarction, endothelial cells were injected into common carotid artery in rabbits. As control examination, blood clots or arachidonic acid was injected into common carotid artery in rabbits.<BR>The subjects were 72 healthy persons, 23 hemodialysis patients and the male rabbits which were 3-4 kg of body weight.<BR>Results obtained were as follows;<BR>1) With Giemsa staining, cells to be thought as endothelial cells were shown, as a rule, polygonal shape, often with folded or rolled suggesting a marked flatness. The membrane of the cell was stained with Ruthenium Red and by the fluorescent staining, and we could observe the intence fluorescence in the cell with using anti-human factor VIII and anti-human α<SUB>2</SUB>-macroglobulin serum.<BR>2) When blood is taken with a syringe, endothelial cells may be detached and taken together. To prevent it, blood was taken from arterial side tube of extracorporeal circulation in hemodialysis patients. These samples, also showed the presence of cells suggesting the endothelial cells. The average of the circulating cell numbers in healthy subjects was 1.59 cells/9 μlPRP, and that of hemodialysis patients was 1.29 cells/9 μlPRP, showing no significant difference between healthy subjects and hemodialysis patients.<BR>3) By injections of endothelial cells into common carotid artery in rabbits, cerebral embolisms could not be observed. But, by injections of blood clots or arachidonic acid into common carotid artery, cerebral embolisms could be observed. It was suggested from above results that the increasing of circulating endothelial cells cannot cause embolism by itself.
- 一般社団法人 日本脳卒中学会の論文
一般社団法人 日本脳卒中学会 | 論文
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