Evaluation of a rat model for disseminated intravascular coagulation developed by infusion of lipopolysaccharide.
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概要
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A rat model of disseminated intravascular coagulation was developed by utilizing various routes of lipopolysaccharide (LPS) administration and comparisons were made. Either 2.0, 5.0, 10.0mg/kg of LPS was administered to the rats intratracheally (i.t.), intraperitoneally (i.p.), intravenously (b.i.) or by 24hr. continuous intravenous infusion (c.i.). Coagulation disturbances and the degree of organ dysfunction were then examined. Platelets counts ware significantly decreased in the b.i. and c.i. groups, while there was no significant change in the i.t. and i.p. groups. Plasma fibrinogen levels were significantly decreased only in the c.i. group and the antithrombin III level was decreased in the b.i. and c.i. groups as compared to the saline administered control. Comparison of fibrinogen and antithrombin III levels among the groups revealed a significant decrease in the c.i, as compared to the groups. The degree of liver dysfunction, expressed by GOT, and renal dysfunction, expressed by BUN, were significantly greater in the b.i. and c.i. groups. These changes were most significant in the c.i. group at an LPS dose of 5mg/kg. In addition, total protein levels and albumin levels were significantly decreased in the c.i. group. Histopathological changes were also most remarkable in the c.i. group, which showed hemorrhage and migration of neutrophils and mononuclear cells in the lung, leukocyte migration, edema and midzonal and centrilobular necrosis in the liver, fibrinous deposits in glomerulus, leukocyte migration, and necrosis in the kidney. These results indicate that the coagulation responses, as well as organ dysfunction in the liver and kidney, were most prominent in the c.i, group at the same LPS challenge dose. We thus conclude that this model is highly appropriate as a rat septic DIC and organ dysfunction model.
- 一般社団法人 日本救急医学会の論文
一般社団法人 日本救急医学会 | 論文
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