Morphologic changes in the lymphatics of rat abdominal dermis after thermal injury using three-dimensional reconstruction images.
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概要
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To elucidate the progressive pathology of burned dermal lymphatics, present study shows three-dimensional reconstruction images of the lymphatics in rat dermis subjected second degree scald burns were constructed, and the volume of the lymphatics per skin area measured. Local scald burns were inflicted by applying 70°C hotwater for 5 seconds on the abdominal wall of female Wistar rats. Burned skins were harvested at 1, 2, 4, 8, and 12 hours, and 1, 2, 4, 7, and 14 days postburn. Rats were anesthetized with ethylether during any intervention. Specimens were embedded in epoxy-resin. Semithin 300 sections 2μm were serially cut from each resin block. Every third section was examined and photographed. Each lymphatic image in a series of 100 pictures were taken with a CCD camera, and three-dimensional reconstruction figures were made by computer (using OZ soft; RIZE company Japan). Dermal lymphatic volumes were also measured. Unburned rats were prepared as controls. In normal dermis several lymphatics running at the sebaceous gland level, to which a few subepidermal lymphatics descended were seen. The lymphatics developed several hours postburn, markedly at 4 hours postburn, to well-expanded subepidermal lymphatics, with a lymphatic network that formed a plexus in the mid-dermis, and further descending lymphatics. On and after 1 day postburn, lymphatics in the upper dermis gradually disappeared and the continuity of dermal lymphatics decreased. Consequently, the structure of lymphatics in the mid/deep dermis remained from 4 to 14 days postburn. Volumes expanded significantly from 1 hour to 2 days (the volumes between 4-12 hours increased to 7∼9-fold that of normal animal). Measurement of burned dermal lymphatic volume is thought to reflect the extent of lymphatic distention, and these results correlate with previous physiological findings. This study outlined the actual morphological changes in dermal lymphatics over the postburn period.
- 一般社団法人 日本救急医学会の論文
一般社団法人 日本救急医学会 | 論文
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