Effect of Low Calcium Diet on Messenger Ribonucleic Acid Levels of Calbindin-D28K of Intestine and Shell Gland in Laying Hens in Relation to Egg Shell Quality.

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We investigated the effects of short-term feeding low calcium diet on egg shell quality (specific gravity, relative weight of egg shell, egg shell thickness) and mRNA levels of calbindin-D28K (CaBP-D28K) in the shell gland and intestine. After feeding low calcium diet (0.5%), hens laid fragile eggs with low shell quality. Plasma calcium concentration also decreased significantly after feeding of low calcium diet. When the hens received a moderate calcium diet (3.5%) after low calcium diet for 5 days, shell quality was improved and plasma calcium concentrations increased. Levels of CaBP-D28K mRNA in the shell gland did not change after feeding low calcium diet for 5 days. Levels of CaBP-D28K mRNA in the intestine of the low calcium diet group significantly increased 2 fold when compared to those of the control group. We infer that low calcium concentration in plasma may stimulate parathyroid and 1, 25(OH)2D3 production in the kidney and induce CaBP-D28K mRNA expression in the intestine. It is supposed that a low calcium diet influences egg shell quality, plasma calcium concentration and intesti-nal CaBP-D28K gene expression by calcium homeostasis. In the shell gland, however, the levels of CaBP-D28K mRNA may not be influenced by low plasma calcium.
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