Analytical Method for Residual Sulfonamides in Various Tissues of Chicken by High-performance Liquid Chromatography.

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A simple, rapid and sensitive analytical method for determination of sulfamonomethoxine (SMM), sulfadimethoxine (SDM) and sulfaquinoxaline (SQ) in various tissues of chicken (blood, muscle, liver, kidney, ovary and fat) was investigated by high-performance liquid chromatography (HPLC).The extraction of sulfonamides from the samples was performed using 80% (v/v) acetonitrile solution and n-hexane to minimize the amount of fat contents. The column chromatography was carried out using alumina acidic, basic (B), neutral and silica gel. The alumina B column was most effective to remove impurities involved in fats and proteins from extract. The recoveries of sulfonamides were from 97.1 to 104.0% with 30ml of 90% (v/v) acetonitrile solution in this procedure. The refined samples containing sulfonamides were labeled with fluorescamine and were applied to HPLC column packed with LiChrosorb RP-18.The average recovery of SMM, SDM and SQ from various tissues of chicken fortified at 1.0ppm (5μg/sample 5g) were from 85.2 to 101.9% with coefficient of variation within 4.9%. The detection limits were 2 ppb for SMM and SDM, and 4 ppb for SQ.
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