Influence of Fasting and Refeeding on Protein, DNA, RNA and mRNA Contents in the Liver of Chicks.

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The influence of fasting and refeeding on protein, DNA, RNA and mRNA contents in the liver of chicks was investigated.Total mRNA was purified from total RNA by using modified Poly (U)-Sepharose affinity chromatography. The mRNA content was determined by measuring the absorbance at 260nm and polyadenylic acid (5') was used as a standard.Total protein, DNA and RNA contents in the liver were reduced by fasting and were increased by refeeding although they remained to be significantly lower than the control. The relative amount of mRNA was unchanged by both fasting and refeeding. Food deprivation for 3 days decreased total mRNA content which was recovered by the following 3-day-refeeding up to the control level.The significant influence of fasting and refeeding was not observed in RNA: protein ratio. This unchanged ratio suggested that, at least, food deprivation and refeeding did not change the relative concentration of hepatic RNA, which can be regarded as the site of protein synthesis. Although there was no significant difference in mRNA: DNA ratio between 3-day-fasting and control groups, the ratio in the refed group was higher than that in the age-matched control group. The apparent increase in mRNA: DNA ratio by refeeding would result from the lower DNA content than control, and this result could lead the possibility of enhancing the transcriptional step and/or the stability of mRNA molecules by fasting and refeeding.
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