Culture of Japanese Quail Embryos from the Blastoderm Stage to Hatch in Chicken Egg Shells.

概要

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The blastodermal embryos of Japanese quail from freshly laid eggs were cultured to hatch in chicken egg shells with the blunt end removed.The culture was conducted using three methods. In method 1, the entire contents of a freshly laid egg were transferred into a chicken egg shell. In method 2, one ml of chicken thin albumen was added after transfer of the entire egg contents. In method 3, the egg yolk was transferred into a chicken egg shell containing about 30 ml of chicken thin albumen. Volume of chicken thin albmen was adjusted to about 8 ml after 2.5 days of culture. The rates of hatching of the cultured embryos were 17.6%, 13.5% and 11.4% for methods 1, 2 and 3, respectively. In all methods the viability of embryos decreased after 14 days of culture. About half of hatchlings had physical troubles in legs and died within one week after hatching. But remaining half grew normally to adults. Method 1 showed the highest hatchability among the three methods and its procedure was simple, but many abnormal embryos were observed. In method 3, there was no abnormal embryo but the hatchability was low.The present study shows that it is possible to culture the blastodermal embryos of Japanese quail to hatch using chicken egg shells as culture vessels.
日本家禽学会の論文