Effects of testicular grafts on the development of ovary in female chick embryo.

概要

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In order to develop the method of artificial control of sexual differentiation in chicken, we have investigated the effects of testicular grafts on the development of reproductive organs in female embryo. Testes from 13-day-old chick embryo were grafted into the extraembryonic coelom of 3-day-old embryo. The recipients were sacrificed on 13 or 16 days of development, and their gonads and Mullerian ducts were examined. 2/3 of female embryo in which the grafted testes were vascularized, atrophy of ovaries in both side and regression of the Mullerian ducts was prominent. Typical tubular structure of testis was observed in some histological view of the atrophic ovaries. Amounts of estradiol-17β produced during in vitro incubation of the atrophic ovaries were less than those of normal ovaries, while the production of testosterone was not affected. In conclusion, it is suggested that the developing testes secrete substances which cause the differentiation of gonads as well as the regression of the Mullerian ducts.
日本家禽学会の論文