Developmental changes in free amino acid and low molecular weight peptide of blood from growing cockerel.
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概要
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The present study was carried out to clarify changes with aging in free amino acid and low molecular weight peptide of blood from cockerel. Paper electrophoresis was employed to analyze and identify the specific substances in erythrocytes from cockerel. Highspeed liquid chromatography was also used for quantitative determination of free amino acid and low molecular weight peptide in blood from cockerel. Blood samples were collected from wing vein of White Leghorn cockerels aging from 1 to 30 weeks, adult male Japanese quail and adult domestic drakes into heparinized tubes.On the cathode side in paper electrophorograms, the two specific bands of free amino acid were observed in erythrocytes from cockerel, while not found in them from adult male Japanese quail and domestic drake. One, placed on the anode side, of the specidic bands was identified to be carnosine and the other band was to be anserine based on relative mobility and ninhydrine and diazo reactions.Concentrations of the two specific substances were measured by highspeed liquid chromatography. The concentrations, in terms of carnosine equivalent, in erythrocytes from cockerel were significantly higher than those in plasma from cockerel and in erythrocytes and plasma from adult male Japanese quail and domestic drake.Ornithine was not detected in erythrocytes from cockerel after 5 weeks of age. Aspartate concentration decreased as the age progressed, being undetectable at 30 weeks of age. Threonine concentration decreased until 20 weeks of age and increased during 20 to 30 weeks of age. In contrast, tyrosine and phenylalanine appeared first at 5 weeks of age and then increased until 30 weeks of age. On the other hand, no remarkable change was found on free amino acid concentration in plasma from cockerel relating to the age.Pattern similarities of free amino acid among erythrocytes and plasma from cockerel, adult male Japanese quail and domestic drake were calculated. Free amino acid composition in erythrocytes from cockerel changed remarkably with aging. Pattern similarity of free amino acid between 1 and 2 weeks of age, and 20 and 30 weeks of age of cockerel was high, being 0.995 and 0.984 respectively. However, pattern similarity between 1 or 2 weeks and 30 weeks of age of cockerel showed a relatively low ratio, 0.846 or 0.828 respectively. On the other hand, free amino acid composition in plasma from cockerel did not change in relation to aging, and no significant difference was found among free amino acid compositions in plasma from cockerel, adult male Japanese quail and domestic drake.
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