Histochemical demonstration of estrogen and progesterone binding sites in the shell gland of immature, laying and non-laying hens.

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The localization of the estradiol and progesterone binding sites in the shell gland under various functional conditions was examined histochemically using FITC conjugated BSA-ste-roids. The bindings of these steroid derivatives were observed specifically in the mucousExplanation of FiguresFigs.1-6. Frozen sections of the shell gland tissue incubated with steroid-BSA-FITC.E: mucous epithelium, LP: lamina propria, S : space between mucous plicaeFig.1. E2-BSA-FITC treated section of laying hen's shell gland. Fluorescence induced by FITC can be observed in the mucous epithelium (green in color). ×200.Fig.2. Same section as in Fig.1. ×400.Fig.3. P-BSA-FITC treated section of laying hen's shell gland. ×400.Fig.4. E2-BSA-FITC treated section of immature hen's shell gland. ×400.Fig.5. E2-BSA-FITC treated section of non-laying hen's shell gland. Arrows show non-specific fluorescence (orange in color). ×400.Fig.6. Control section for E2-BSA-FITC treatment in laying hen's shell gland. ×400.epithelial cells, but not in the other tissues of the shell gland of laying hens. Weaker fluorescence of these steroid derivatives was observed only in the apical portion of the mucous epithelial cells in immature and non-laying hens.
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