The efficacy of glucose and electrolyte solutions in preserving the structure of fowl spermatozoa after freezing and thawing.
スポンサーリンク
概要
- 論文の詳細を見る
In a series of morpho-physiological studies on freezing injuries in fowl spermatozoa, the present study dealt with the influence of electrolyte and non-electrolyte on structure of unfrozen and frozen-thawed spermatozoa. Fowl spermatozoa were suspended in electrolyte (sodium citrate, sodium chloride, potassium chloride and calcium chloride) solutions and in a non-electrolyte (glucose) solution, and incidences of crooked-necked spermatozoa (CNS) and abnormal acrosomes were investigated to ascertain whether the increased incidence of CNS during the freeze-thawing procedure might be caused by the high concentration of chloride ion in media, and whether the acrosomal damage might be causes by increased osmotic pressure in media during the freezing process. The incidences of CNS occurring during the freeze-thawing process tended to be comparatively larger in spermatozoa suspended in solutions containing chloride ions than in those in solutions without chloride ion, although there were no significant differences between the former and the latter.The deterioration in acrosomes due to freeze-thawing was evident in all samples, but were significantly higher in the glucose solution than in other solutions.From these results, it is speculated that increased incidence of CNS during freeze-thawing is related to the high concentration of chloride ion in the media, and that the high osmotic pressure which is produced by concentration of solutes in media may cause the acrosomal damage.
- 日本家禽学会の論文
日本家禽学会 | 論文
- ニワトリ MHC クラスIcDNA プローブを用いたニホンウズラゲノミック DNA の ECL 法による RFLP 分析
- 各種家禽の赤血球中のカルノシンおよびアンセリンの検出〔英文〕
- 鶏の加齢と血液中の遊離アミノ酸および低分子ペプチド組成との関係
- ニワトリヒナのファブリシウス嚢におけるグルココルチコイド誘導性のアポトーシスに関する免疫組織化学的検討
- 鶏の体脂肪蓄積に及ぼす分離大豆タンパク質及びカゼインの影響