The Effect of Adding Fractionated Egg Yolk on Motility, Percent Motile Sperm and Fertility of Chicken Spermatozoa

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The effect of adding egg yolk fractionated by chloroform-methanol (2:1) was studied with chicken spermatozoa stored 24 and 48 hours at 2°C.1. The addition of freeze-dried egg yolk (50mg/ml) to a phosphate buffer containing antibiotic resulted in high motility and percent motile sperm but in lowered fertility as compared to a control (phosphate buffer).2. The water soluble portion of a chloroform-methanol insoluble fraction was highly effective in maintaining motility and percent motile sperm but it lowered fertility.3. The addition of a chloroform-methanol soluble fraction (50mg/ml) to a phosphate buffer had no effect on motility, percent motile sperm and fertility.
日本家禽学会の論文