:I. Effects of Several Factors on Reversible Inactivation
スポンサーリンク
概要
- 論文の詳細を見る
The motility of mammalian spermatozoa, generally, increases with the rise in temperature and is most active at the body temperature. On the other hand, it was reported that cock spermatozoa were reversibly inactivated by the temperature of 40°C in certain saline media.This study is continued in order to make clear the process, causes and mechanism of the reversible inactivation of cock spermatozoa. This paper describes the conditions under which the reversible inactivation occurs and factors which effect it. For comparison, ram spermatozoa were also investigated, in addition to cock spermatozoa.(1) Cock spermatozoa were inactivated at 40°C in saline media. This inactivation was not permanent and the motility was again restored when the temperature was lowered to about 20°C. However, ram spermatozoa were most motile at 40°C and did not show inactivation until they lost motility after several hours in the same media.Washed cock spermatozoa were reversibly inactivated more quickly than the diluted spermatozoa at 40°C in saline media. Undiluted cock spermatozoa were not reversibly inactivated at 40°C and preserved the vigorous motility more than two hours at this temperature. They were reversibly inactivated at 45°C but undiluted ram spermatozoa were not reversibly inactivated at the same tempeature. At 50°C both cock and ram spermatozoa died within several miuntes.(2) The inactivation time (the time reqired to reversible inactivation) of centrifugally washed cock spermatozoa was shorter than that of unwashed spematozoa in the same dilution ratio. This fact may be due to the effect of washing and not due to the effect of centrifugation.(3) In the saline media which inactivated them within very short time at 40°C, though their inactivation times were prolonged, cock spermatozoa were reversibly inactivated even at 30°C.(4) The inactivation time and motility of cock spermatozoa were inversely related to dilution ratio and storage time. This relation may be due to senescence of spermatozoa.(5) Cock spermatozoa inactivated at 40°C in air and in O2 could not be reactivated by filling with O2 and by shaking, though their motility was restored immediately when the temperature was lowered to about 20°C.As for spermatozoa inactivated at 40°C in CO2, reactivation needs the procedure of both cooling of temperature and supply of air.(6) When blood plasma, egg white and refined egg albumen were added to the media, the inactivation time of washed cock spermatozoa was prolonged. It may be due to the protective action of macromolecules especially albumen.On the other hand, additon of phosphate buffer reduced the inactivation time.When supernatant fluids from homogenates of six regions of hen oviduct were added, the inactivation time did not vary significantly.
- 日本家禽学会の論文
日本家禽学会 | 論文
- ニワトリ MHC クラスIcDNA プローブを用いたニホンウズラゲノミック DNA の ECL 法による RFLP 分析
- 各種家禽の赤血球中のカルノシンおよびアンセリンの検出〔英文〕
- 鶏の加齢と血液中の遊離アミノ酸および低分子ペプチド組成との関係
- ニワトリヒナのファブリシウス嚢におけるグルココルチコイド誘導性のアポトーシスに関する免疫組織化学的検討
- 鶏の体脂肪蓄積に及ぼす分離大豆タンパク質及びカゼインの影響