Cryopreservation of semen from onagadori,the Japanese long-tailed fowl.
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概要
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Six independent trials were conducted sequentially with semen from the Japanese Long-Tailed Fowl to determine the optimal pre-freeze and freeze procedures for maximum recovery of spermatozoal motility. Highest levels of pre-freeze motility (4.3±0.2) were obtained when semen was diluted 1:3 with Beltsville Poultry Semen Extenter, cooled for 1.0h at 5°C, then 4% dimethylsulfoxide (DMSO) added in bulk, and the sample equilibrated an additional 1.0h at 5°C. Highest levels of post-thaw motility (3.5±0.0) were obtained when semen was first frozen at 1.0°C/min from +5°C to -20°C, then exposed to liquid nitrogen vapor temperatures between -81° and -154°C for 10mim, before plunging into liquid nitrogen.
- 日本家禽学会の論文
日本家禽学会 | 論文
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