Effect of Cock Serum on Motility, Deformity and Fertility of Ejaculated Cock Spermatozoa

概要

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Present studies were carried out to clarify the effects of cock serum on the motility, deformity and fertility of cock spermatozoa.Semen was collected from 10-15 roosters by the method of abdominal massage. The semen was centrifuged for 5 minutes at a speed of 3, 000r.p.m, and the spermatozoa were washed twice with phosphate buffer (δ=0.615, pH=7.17) and resuspended in cock serum diluted at different rates with the phosphate buffer. Observation and insemination were performed just after treatment or after storage at 0°C for one to six days.The results obtained are as follows:(1) When the washed spermatozoa were resuspended in phosphate buffer, motility of spermatozoa was extremely suppressed. However, fertility was not affected.(2) Motility of washed spermatozoa, which was suppressed by phosphate buffer, was wholly regained with addition of cock serum.(3) Cock serum diluted 6- or 8-fold with phosphate buffer was highly effective for the storage of washed spermatozoa, compared to undiluted cock serum and phosphate buffer.(4) Fertility of washed spermatozoa stored for 1 day at 0°C was higher in cock serum diluted 6- or 8-fold (36-81%) than in phosphate buffer (0-24%) and undiluted cock serum (0%).These results show that the addition of cock serum in a low concentration to phosphate buffer is useful for the storage of cock spermatozoa in vitro.
日本家禽学会の論文