Single Cell Protein Production from Liquefied Petroleum Gas

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A propane and butane-utilizing bacterial strain, capable of growth at a comparatively high temperature with a specific growth rate of 0.12hr-1, has been isolated from soil samples by enrichment culture. The optimum temperature and pH range for the growth of the isolate, designated SB-1701, were 35∼40°C and 5.7∼6.7, respectively.Cultivation was carried out with a mixed gas of 20% n-butane and 80% air, using only inorganic salts as the medium. The optimum medium composition which contained trace metallic salts was determined to obtain high productivity. Continuous culture was maintained at a steady state for more than 400 hours in a 20-liter jar fermenter during which the productivity was 1.4g cell/l•hr. The yield based on propane was 0.95g cell/g propane and the yield based on oxygen was 0.41g cell/g oxygen. All experiments were carried out aseptically.SB-1701 has been identified as a new strain of the genus Flavobaclerium.The crude protein content and the amino acid profile of SB-1701 were determined.
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