Development to the Blastocyst Stage of Pig Embryos Cultured in the Amniotic Fluid of Developing Chick Embryos.
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概要
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Early-stage pig embryos were collected from superovulated donor gilts (60-72 hr post hCG injection) and co-cultured in modified Brinster medium for ovum culture (mBMOC-2) + porcine oviduct epithelial cells (POEC) or in the amniotic fluid of a developing chick embryo (CEAm). The development rate to the compacted morulae or blastocyst stage was significantly higher (P<0.01) in embryos co-cultured in CEAm (60.0%) than those co-cultured in mBMOC-2 + POEC (18.0%). The results indicate that CEAm could be used for production of compacted morulae or blastocysts from early-stage pig embryos. This culture system could also be valuable in situations where a CO<SUB>2</SUB> controlled incubator is not available.
- 公益社団法人 日本獣医学会の論文
公益社団法人 日本獣医学会 | 論文
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