An "affinity" method for preparing polypeptides enriched in the collagen-associated Ehrlich chromogen.

スポンサーリンク

概要

• 論文の詳細を見る

• The collagen-associated compound which reacts at room temperature with acid dimethylaminobenzaldehyde (Ehrlichs reagent) also reacts in acid with aryl-diaz-onium reagents. We have developed a convenient method for isolating polypeptides associated with the Ehrlich chromogen from collagen digests utilizing diazotized arylamino-cellulose supports. These peptides, in which the Ehrlich chromogen is 'labeled' with yellow diazo color, constitute less than 0.5% of the collagen, and have amino acid patterns similar to those around the cross link regions. The chromogen is not identical with pyridinoline, also thought to be a polyvalent cross link.

• 社団法人 日本生化学会の論文

著者

• Scott John

  Department Of Biochemistry Trinity College Dublin Dublin

• Hughes Eira

  Department of Chemical Morphology, Chemistry Building

• Shuttleworth Adrian

  Department of Medical Biochemistry, University of Manchester

関連論文

• Measurements of sub-nanomolar concentrations of unmetabolised folic acid in serum
• Analysis of the MTHFR 1298A→C and 677C→T polymorphisms as risk factors for neural tube defects
• An "affinity" method for preparing polypeptides enriched in the collagen-associated Ehrlich chromogen.

スポンサーリンク