BINDING OF DYE IONS BY PLASMA PROTEINS IN DISEASES

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概要

• 論文の詳細を見る

• Spectrophotometric analysis of the binding of dye by proteins has yielded the following results. 1. Human serum albumin shows two step combination reaction with methyl orange, while horse, bovine, hen, and rabbit serum albumins show only one step reaction. 2. Albumin concentration and therefore the A/G ratio of normal and various pathological sera can be determined fairly accurately by spectrophotometric determination of bound dyes except in the case of hepatic insufficiency and nephrosis. 3. Serum proteins in severe hepatic insufficiency bind less dye ions than can be accounted for on the basis of albumin concentration. The author expresses his cordial thanks to Prof. K. Kodama and Assist. Prof. H. Yoshikawa for their kind guidance and also to Dr. H. Hirai and Mr. K. Shimao for their helpful advice and valuable discussion throughout the course of these investigations. The author is greatly indebted to Dr. K. Sakaguchi, Chief of the First Tokyo National Hospital, Dr. K. Miyoshi and Dr. T. Sasakiin Tokyo University Hospi-tal, and Dr. F. Yanagisawa for their kind supply of normal and pathological sera used in this study.

• 社団法人 日本生化学会の論文

著者

• KUSUNOKI TOMOICHI

  Department of Biochemistry, Faculty of Medicine, Tokyo University

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• BINDING OF DYE IONS BY PLASMA PROTEINS IN DISEASES

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