2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, Induces Accelerated Production of Chemokines in HL-60 Cells

スポンサーリンク

概要

• 論文の詳細を見る

• 2-Arachidonoylglycerol is an endogenous ligand for the cannabinoid receptors (CB 1 and CB 2). Previously, we provided evidence that 2-arachidonoylglycerol, but not anandamide (N-arachidonoylethanolamine), is the true natural ligand for the cannabinoid receptors. In the present study, we examined in detail the effects of 2-arachidonoylglycerol on the production of chemokines in human promyelocytic leukemia HL-60 cells. We found that 2-arachidonoylglycerol induced a marked acceleration in the production of interleukin 8. The effect of 2-arachidonoylglycerol was blocked by treatment of the cells with SR 144528, a cannabinoid CB 2 receptor antagonist, indicating that the effect of 2-arachidonoylglycerol is mediated through the CB 2 receptor. Augmented production of interleukin 8 was also observed with CP 55940, a synthetic cannabinoid, and an ether-linked analog of 2-arachidonoylglycerol. On the other hand, neither anandamide nor the free arachidonic acid induced the enhanced production of interleukin 8. A similar effect of 2-arachidonoylglycerol was observed in the case of the production of macrophage-chemotactic protein-1. The accelerated production of interleukin 8 by 2-arachidonoylglycerol was observed not only in undifferentiated HL-60 cells, but also in HL-60 cells differentiated into macrophage-like cells. Noticeably, 2-arachidonoylglycerol and lipopolysaccharide acted synergistically to induce the dramatically augmented production of interleukin 8. These results strongly suggest that the CB 2 receptor and its physiological ligand, i.e., 2-arachidonoylglycerol, play important regulatory roles such as stimulation of the production of chemokines in inflammatory cells and immune-competent cells. Detailed studies on the cannabinoid receptor system are thus essential to gain a better understanding of the precise regulatory mechanisms of inflammatory reactions and immune responses.

• 社団法人 日本生化学会の論文

著者

• Oka Saori

  Faculty Of Pharmaceutical Sciences Teikyo University

• Waku Keizo

  Faculty Of Pharmaceutical Science Teikyo University

• Gokoh Maiko

  Faculty Of Pharmaceutical Sciences Teikyo University

• Sugiura Takayuki

  Faculty Of Pharmaceutical Sciences Teikyo University

• Kishimoto Seishi

  Faculty Of Pharmaceutical Sciences Science University Of Tokyo

• Kobayashi Yuriko

  Faculty Of Applied Biological Sciences Gifu University

関連論文

• 2-Arachidonoylglycerol Enhances the Phagocytosis of Opsonized Zymosan by HL-60 Cells Differentiated into Macrophage-Like Cells(Highlighted paper selected by Editor-in-chief,Biochemistry)
• Depolarization-induced Rapid Generation of 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, in Rat Brain Synaptosomes
• Physiological Functions of 2-Arachidonoylglycerol
• Endogenous Cannabinoid Receptor Ligand Induces the Migration of Human Natural Killer Cells
• New Perspectives in the Studies on Endocannabinoid and Cannabis : 2-Arachidonoylglycerol as a Possible Novel Mediator of Inflammation
• 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, Induces Accelerated Production of Chemokines in HL-60 Cells
• Inhibition of Cytosolic Phospholipase A_2 Suppresses Production of Cholesteryl Ester through the Reesterification of Free Cholesterol but not Formation of Foam Cells in Oxidized LDL-Stimulated Macrophages(Highlighted paper selected by Editor-in-chief,Bioc
• The Effect of Cadmium on Arachidonate Release in Rabbit Alveolar Macrophages (Proceedings of the 14th Symposium on Environmental Pollutants and Toxicology)
• "A-11 Determination of Aflatoxin B_1 : Human Serum Albumin Adducts by Sandwich ELISA.
• Activation by 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, of p42/44 Mitogen-Activated Protein Kinase in HL-60 Cells
• Occurrence of a Novel Cannabimimetic Molecule 2-Sciadonoylglycerol(2-Eicosa-5', 11', 14'-trienoylglycerol) in the Umbrella Pine Sciadopitys verticillata Seeds
• Roles of C-Terminal Processing, and Involvement in Transacylation Reaction of Human Group IVC Phospholipase A2 (cPLA2γ)
• Acyltransferases and Transacylases Involved in Fatty Acid Remodeling of Phospholipids and Metabolism of Bioactive Lipids in Mammalian Cells
• EFFECT OF CADMIUM IONS ON PHOSPHOLIPID METABOLISM DURING THE EXCITABILITY OF EMBRYONIC CHICKEN HEART VENTRICULAR CELLS
• Ca^-DEPENDENT ACTION POTENTIALS AND PHOSPHATIDYLINOSITOL METABOLISM IN EMBRYONIC CHICKEN HEART VENTRICULAR CELLS. EFFECT OF CADMIUM IONS OR CALCIUM ANTAGONISTS
• Effects of Several Heavy Metals on the Metabolism of Arachidonic Acid in Rabbit Alveolar Macrophages (Proceedings of the 18th Symposium on Toxicology, and Environmental Health)
• Effect of Cadmium Exposure on the Lipid Composition of Rat Testis in Vivo. (Regular Presentations) (Proceedings of the 9th Symposium on Environmental Pollutants and Toxicology)
• STUDIES ON THE PHOSPHOLIPID METABOLISM OF RAT LIVER AND KIDNEY AFTER LONG TERM CADMIUM ADMINISTRATION
• 2-Arachidonoyl-sn-glycero-3-phosphoinositol : A Possible Natural Ligand for GPR55
• Protection by Zinc against Cadmium Toxicity in Rat Liver and Kidney (Regular Presentations) (Proceedings of the 11 th Symposium on Environmental Pollutants and Toxicology)
• Accumulation of Inositol 1, 4, 5-Trisphosphate in the Rat Brain as a Result of Long-term Administration of Lithium
• Lysophosphatidylinositol induces rapid phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor 2 in HEK293 cells expressing GPR55 and IM-9 lymphoblastoid cells
• Is the Cannabinoid CB1 Receptor a 2-Arachidonoylglycerol Receptor? Structural Requirements for Triggering a Ca^ Transient in NG108-15 Cells
• Effects of Cadmium on the Metabolism of Fatty Acid in Rat Liver (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
• The Role of Zinc in Fatty Acid Metabolism in Rat Liver : Effects of Zinc Deficiency and Cadmium Administration (Regular Presentations) (Proceedings of the 15 th Symposium on Environmental Pollutants and Toxicology)
• The Effect of Cadmium-Microsome High-Molecular-Weight Protein Complex on Some Enzyme Activity (Proceedings of the 12 th Symposium on Environmental Pollutants and Toxicology) (Regular Presentations)
• Cannabinoid Receptors and Their Endogenous Ligands
• 11-7 QTL Analyses of Primary Root Length of Arabidopsis thaliana using four sets of Recombinant Inbred Lines under control condition
• 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, Induces Accelerated Production of Chemokines in HL-60 Cells
• Activation by 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, of p42/44 Mitogen-Activated Protein Kinase in HL-60 Cells.
• Is the Cannabinoid CB1 Receptor a 2-Arachidonoylglycerol Receptor? Structural Requirements for Triggering a Ca2+ Transient in NG108-15 Cells.
• QTL Analyses of anther dehiscence in rice (Oryza sativa L.)

もっと見る 閉じる

スポンサーリンク