Elevation of Plasma Membrane Permeability on Laser Irradiation of Extracellular Latex Particles

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In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd: YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 μm in diameter) and mouse fibrosarcoma (Meth-A) cells. After irradiation, membrane permeability was evaluated by flow cytometric assaying using propidium iodide (PI) and fluorescein diacetate (FDA). The proportion of permeabilized-resealed cells was affected by changes in the light intensity (_??_780 mW/cm2), the irradiation time (_??_240 s), and/or the particle concentration (_??_109 particles/ml). The permea-bility persisted up to 20min after light irradiation. Near the sites of individual particles, the permeability of the cell membrane is modified, probably due to localized temperature changes. These results suggest that this laser-induced permeabilization strategy constitutes a new means of delivering exogenous materials into living cells.
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