Contribution of Conserved Amino Acids at the Dimeric Interface to the Conformational Stability and the Structural Integrity of the Active Site in Ketosteroid Isomerase from Pseudomonas putida Biotype B

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概要

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• Ketosteroid isomerase (KSI) from Pseudomonas putida biotype B is a homodimeric enzyme catalyzing an allylic isomerization of Δ5-3-ketosteroids at a rate of the diffusion-controlled limit. The dimeric interactions mediated by Arg 72, Glu 118, and Asn 120, which are conserved in the homologous KSIs, have been characterized in an effort to investigate the roles of the conserved interface residues in stability, function and structure of the enzyme. The interface residues were replaced with alanine to generate the interface mutants R72A, E118A, N120A and E118A/N120A. Equilibrium unfolding analysis revealed that the ΔGUH2O values for the R72A, E118A, N120A, and E118A/N120A mutants were decreased by about 3.8, 3.9, 7.8, and 9.5 kcallmol, respectively, relative to that of the wild-type enzyme. The interface mutations not only decreased the kcat/KM value by about 8- to 96-fold, but also increased the KD value for d-equilenin, a reaction intermediate analogue, by about 7- to 17.5-fold. The crystal structure of R72A determined at 2.5 Å resolution and the fluorescence spectra of all the mutants indicated that the interface mutations altered the active-site geometry and resulted in the decreases of the conformational stability as well as the catalytic activity of KSI. Taken together, our results strongly suggest that the conserved interface residues contribute to stabilization and structural integrity of the active site in the dimeric KSI.

• 社団法人 日本生化学会の論文

著者

• Yun Young

  National Institute Of Health Sciences

• NAM Gyu

  National Research Laboratory of Protein Folding and Engineering

• HA Nam-Chul

  National CRI Center for Biomolecular Recognition Pohang University of Science and Technology

• JANG Do

  National Research Laboratory of Protein Folding and Engineering

• HONG Bee

  National Research Laboratory of Protein Folding and Engineering

• OH Byung-Ha

  National CRI Center for Biomolecular Recognition Pohang University of Science and Technology

• CHOI Kwan

  National Research Laboratory of Protein Folding and Engineering

• Kim Do-hyung

  National Institute Of Animal Science

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