Structure Determination of the Constitutive 20S Proteasome from Bovine Liver at 2.75 .ANGS. Resolution.

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概要

• 論文の詳細を見る

• The crystal structure of the 20 S proteasome from bovine liver was determined by the molecular replacement method using the structure of the 20 S proteasome from the yeast Sacecharomyces cerevisiae. The initial phases were refined by density modification coupled with non-crystallographic symmetry averaging. The structural model was refined with the program CNS. The final R-factor and Rfree were 0.25 and 0.29, respectively. The constitutive proteasome without any contamination by the immunoproteasome was identified in the crystal structure.

• 社団法人 日本生化学会の論文

著者

• Tsukihara Tomitake

  Institute For Protein Research Osaka University

• Tomisugi Yoshikazu

  The Pharmaceutical Department Kumamoto University

• Mizushima Tsunehiro

  Institute For Protein Research Osaka University

• Unno Masaki

  Institute For Protein Research Osaka University

• MORIMOTO Yukio

  Faculty of Science, Himeji Institute of Technology

• Yasuoka Noritake

  Faculty Of Science Himeji Institute Of Technology

• Tanaka Keiji

  The Tokyo Metropolitan Inst Of Med Sci.

• Unno Masaki

  Institute for Protein Research, Osaka University

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