Identification and Characterization of an Antibacterial Peptide of the 26-kDa Protease of Sarcophaga peregrina with Antibacterial Activity.
スポンサーリンク
概要
- 論文の詳細を見る
Previously, we purified a serine protease with a molecular mass of 26 kDa that exhibits potent antibacterial activity from a pupal extract of Sarcophaga peregrina (flesh fly). We divided this protease into 12 peptides and examined their antibacterial activity. A peptide corresponding to residues 155 to 174 (peptide 9) was found to exhibit antibacterial activity comparable to that of the 26-kDa protease. When Escherichia coli was treated with peptide 9, the permeability of both the outer and inner membranes increased, and substrates for β-lactamase and β-galactosidase entered the cells, but β-galactosidase did not leak out of the cells under these conditions. It was suggested that residues 6 to 18 of peptide 9 form an amphiphilic α-helix under hydrophobic conditions with an N-terminal basic loop and then interact with acidic phospholipids in the bacterial membranes.
- 社団法人 日本生化学会の論文
著者
-
Takeuchi Koh
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Tsuji Yumiko
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Homma Ko-ichi
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Shimada Ichio
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Natori Shunji
Natori Industry-sponsored Laboratory The Institute Of Physical And Chemical Research (riken)
-
Aoyama Tomohisa
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Takahashi Hideo
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Nakajima Yuki
Natori Special Laboratory RIKEN
関連論文
- Analyses of the Interaction between Lipid A and an Antibacterial Peptide, Sarcotoxin IA
- Molecular Basis of the High-Affinity Activation of Skeletal Ryanodine Receptor Ca^-Release Channel by Imperatoxin A
- Three-dimensional Solution Structure of Calcium Channel Activator : Impreratoxin A Determined by NMR Spectroscopy
- Solution Structure of Hanatoxin1, Gating Modifier of Drk1 Potassium Channel
- Binding between Azurocidin and Calreticulin : Its Involvement in the Activation of Peripheral Monocytes
- A Long-lived o-Semiquinone Radical Anion is Formed from N-β-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an Insect-derived Antibacterial Substance
- Convenient Peptide Mapping of Immunoglobulin G2b and Differentiation between Leucine and Isoleucine Residues by Mass Spectrometry Using ^2H-Labeled Leucine
- MOLECULAR ANATOMY OF THE HONEYBEE BRAIN (MUSHROOM BODIES)
- Correlation between the Catalase Level in Tumor Cells and Their Sensitivity to N-β-Alanyl-5-S-Glutathionyl-3, 4-Dihydroxyphenylalanine (5-S-GAD)
- Identification and Characterization of an Antibacterial Peptide of the 26-kDa Protease of Sarcophaga peregrina with Antibacterial Activity
- Involvement of EDTP, an Egg-Derived Tyrosine Phosphatase, in the Early Development of Drosophila melanogaster
- CH/π Interaction in the Conformation of Peptides. : A Database Study
- Participation of Two N-Terminal Residues in LPS-Neutralizing Activity of Sarcotoxin IA^1
- Induction of Sarcophaga Central Nervous System Remodeling by 20-Hydroxyecdysone In Vitro
- Stage-Specific Inhibition of Xenopus Embryogenesis by Aprotinin, a Serine Protease Inhibitor
- Involvement of H_2O_2 and O_2^- in the cytotoxicity of N-β-alanyl-5-S-glutathionyl-3,4- dihydroxyphenylalanine (5-5-GAD), a novel insect-derived anti-tumor compound
- Hydrolysis and Synthesis of Substrate Proteins for Cathepsin L in the Brain Basement Membranes of Sarcophaga during Metamorphosis^1
- Crystal Structure of (S)α-Methyltyrosinato-(SSSR)-[(2R,5R,8R,11R)-2,5,8,11-tetraethyl-1,4,7,10-tetraazacyclododecane]cobalt(III) Bromide Perchlorate Dihydrate
- Identification and Characterization of an Anterior Fat Body Protein in an Insect
- Probing Dynamics and Conformational Change of the GroEL-GroES Complex by ^C NMR Spectroscopy
- Identification of a Novel Tissue-Specific Transcriptional Activator FESTA as a Protein That Interacts with the Transcription Elongation Factor S-II
- Cloning of cDNA for Cathepsin B mRNA 3'-Untranslated-Region-Binding Protein (CBBP), and Characterization of Recombinant CBBP
- Nuclear magnetic resonance approaches for characterizing interactions between the bacterial chaperonin GroEL and unstructured proteins(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Identification and Characterization of an Antibacterial Peptide of the 26-kDa Protease of Sarcophaga peregrina with Antibacterial Activity.
- Identification of a Novel Tissue-Specific Transcriptional Activator FESTA as a Protein That Interacts with the Transcription Elongation Factor S-II