Two New Modes of Smooth Muscle Myosin Regulation by the Interaction between the Two Regulatory Light Chains, and by the S2 Domain.
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概要
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Previous studies indicated that single-headed smooth muscle myosin and S1 (a single head fragment) are not regulated through phosphorylation of the regulatory light chain (RLC). To investigate the importance of the double-headedness of myosin and of the S2 region for the phosphorylation-dependent regulation, we made three types of recombinant mutant smooth muscle HMMs with one intact head and an N-terminally truncated head. The truncated head of ΔMID lacked the motor domain, that of Δ(MD+ELC) lacked the motor and essential light chain binding domains, and single-headed HMM had one intact head alone. The basal ATPase activities of the three mutants decreased as the KCIconcentration became less than 0.1M. Such a decrease was not observed for S1, which had no S2 region, suggesting that S2 is necessary for this myosin behavior. This activity decrease also disappeared when RLCs of ΔMD and Δ(MD+ELC), but that of singleheaded HMM, were phosphorylated. When their RLCs were unphosphorylated, the three mutants exhibited similar actin-activated ATPase levels. However, when they were phosphorylated, the actin-activated ATPase activities of ΔMD and Δ(MD+ELC) increased to the S1 level, while that of single-headed HMM remained unchanged. Even in the phosphorylated state, the actin-activated ATPase activities of the three mutants and S1 were much lower than that of wild-type HMM. We propose that S2 has an inhibitory function that is canceled by an interaction between two phosphorylated RLCs. We also propose that a cooperative interaction between two motor domains is required for a higher level of actin activation.
- 社団法人 日本生化学会の論文
著者
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Kato Kazuo
Department Of Cardiology Fujita Health University
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Kojima Shin-ichiro
Department Of Structural Analysis National Cardiovascular Center Research Institute
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Fujiwara Keigi
Department Of Structural Analysis National Cardiovascular Center Research Institute
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Katoh Tsuyoshi
Division Of Chemistry Graduate School Of Science Hokkaido University
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KONISHI Kaoru
Department of Structural Analysis, National Cardiovascular Center Research Institute
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Yazawa Michio
Division Of Chemistry Faculty Of Science Hokkaido University
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Onishi Hirofumi
Department of Electronic Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan
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