Octamer Binding Protein-1 Is Involved in Inhibition of Inducible Nitric Oxide Synthase Expression by Exogenous Nitric Oxide in Murine Liver Cells.

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概要

• 論文の詳細を見る

• Nitric oxide (NO) has diverse effects on immune responses and hepatic functions. In BNL CL.2 cells, the marine embryonic liver cells, inducible nitric oxide synthase (iNOS) mRNA expression appeared after 3h of treatment with IFN-γ and LPS. Interestingly, mRNA and protein expression of iNOS was down-regulated by sodium nitroprusside (SNP) and diethylamine dinitric oxide in a time- and dose-dependent manner, but not by H2O2. TNF-α gene expression was also dramatically reduced by SNP, but IL-6 gene expression was inhibited much less. IFN-γ and LPS-induced chloramphenicol acetyltransferase activity of iNOS promoter constructs was inhibited by SNP- Electrophoretic mobility shift assay showed that SNP inhibited IFN-γ plus LPS-induced Oct-1 binding activity, and the inhibition was reversed by DTT. Mutation in the Oct-1 site completely abolished iNOS promoter activity. In addition, supershift assay and Southwestern analysis demonstrated that the Oct-1 binding activity was inhibited by SNP. Taken together, these results indicate that NO suppresses IFN-γ plus LPS-induced iNOS expression, and that Oct-1 is an important element in this process.

• 社団法人 日本生化学会の論文

著者

• Lee Bok-soo

  Laboratory Of Immunology Korea Research Institute Of Bioscience And Biotechnology

• Pyun Kwang-ho

  Laboratory Of Immunology Korea Research Institute Of Bioscience And Biotechnology

• Kang Hyung-sik

  Laboratory Of Immunology Korea Research Institute Of Bioscience And Biotechnology

• Choi Inpyo

  Laboratory Of Immunology Korea Research Institute Of Bioscience And Biotechnology

• Kim Yong-man

  Laboratory Of Immunology Korea Research Institute Of Bioscience And Biotechnology

• Kim Hwan

  Laboratory ofImmunology, Korea Research Institute of Bioscience and Biotechnology

関連論文

• Octamer Binding Protein-1 Is Involved in Inhibition of Inducible Nitric Oxide Synthase Expression by Exogenous Nitric Oxide in Murine Liver Cells^1
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• Octamer Binding Protein-1 Is Involved in Inhibition of Inducible Nitric Oxide Synthase Expression by Exogenous Nitric Oxide in Murine Liver Cells.

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