Molecular Cloning and Genomic Analysis of Mouse GalNAc .ALPHA.2,6-Sialyltransferase (ST6GalNAc I).

スポンサーリンク

概要

• 論文の詳細を見る

• cDNA clones encoding mouse GalNAc α2, 6-sialyltransferase (ST 6 GalNAc I) were isolated from a mouse submaxillary gland cDNA library. The deduced amino acid sequence of cDNA clones is 526 amino acids in length and has highly conserved motifs among sialyl transferases, sialyl motifs L, S, and VS. The expressed recombinant enzyme exhibited similar substrate specificity to chicken ST 6 GalNAc I. The mouse ST 6 GalNAc I gene was expressed in submaxillary gland, mammary gland, colon, and spleen. The mouse ST 6 GalNAc I gene was also cloned from a mouse genomic library, which was divided into 9 exons spanning over 8 kilobases of genomic DNA. The genomic structure of the mouse ST 6 GalNAc I gene was similar to that of the mouse ST 6 GalNAc II gene. Unlike the ST 6 GalNAc II gene, however, which has a housekeeping gene-like promoter with GC-rich sequences, the ST 6 GalNAc I gene has two promoters and they do not contain GC-rich sequences but contain putative binding sites for tumor-associated transcription factors such as c-Myb, c-Myc/Max, and c-Ets. Analysis of the 5'-RACE PCR products suggested that the mouse ST 6 GalNAc I gene expression is regulated by these two promoters in tissue-specific manners.

• 社団法人 日本生化学会の論文

著者

• Kurosawa Nobuyuki

  Molecular And Cellular Biology Lab. Fac. Of Sci. And Engineering Graduate School Univ. Of Toyama

• KONO Mari

  Molecular Glycobiology, Frontier Research Program, The Institute of Physical and Chemical Research

• INOUE Mio

  Molecular Glycobiology, Frontier Research Program, The Institute of Physical and Chemical Research

• Ikehara Yuzuru

  Division Of Cell Biology Institute Of Life Science Soka University

• Narimatsu Hisashi

  Division Of Cell Biology Institute Of Life Science Soka University

• Tsuji Shuichi

  Molecular Glycobiology Frontier Research Program The Institute Of Physical And Chemical Research(riken)

• Takashima Shou

  Molecular Glycobdology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN)

• Tachida Yuriko

  Molecular Glycobdology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN)

関連論文

• Effect of early eradication on Helicobacter pyloirirelated gastric carcinogenesis in Mongolian gerbils
• Reversibility of Heterotopic Proliferative Glands in Glandular Stomach of Helicobacter pylori-infected Mongolian Gerbils on Eradication
• A polymorphism of C-to-T substitution at -31 IL1B is associated with the risk of advanced gastric adenocarcinoma in a Japanese population
• Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet-cell differentiation from liver metastasis
• Genomic Organization and Transcriptional Regulation of the Mouse GD3 Synthase Gene (ST8Sia I): Comparison of Genomic Organization of the Mouse Sialyltransferase Genes^1
• Molecular Cloning and Genomic Analysis of Mouse GalNAc α2, 6 - Sialyltransferase (ST6GalNAc I)
• Comparative Analysis of the Genomic Structures and Promoter Activities of Mouse Siaα2,3Galβ1,3GalNAc GalNAcα2,6-Sialyltransferase Genes (ST6GalNAc III and IV): Characterization of Their Sp1 Binding Sites^1
• Solitary bone metastasis as the first clinical manifestation in a patient with small bowel adenocarcinoma
• Receptor for Hyaluronan-mediated Motility and CD44 Expressions in Colon Cancer Assessed by Quantitative Analysis Using Real-time Reverse Transcriptase-Polymerase Chain Reaction
• Molecular Cloning and Characterization of a Third Type of N-Glycan α2, 8-Sialy ltransferase from Mouse Lung^1
• Identification of an α2, 6-sialyltransferase induced early after lymphocyte activation
• Minimum Length of Homology Arms Required for Effective Red/ET Recombination
• Comparison of the Enzymatic Properties of Mouse β-Galactoside α2, 6-Sialyltransferases, ST6Gal I and II
• Alpha 1,6-Linked Fucose Affects the Expression and Stability of Polysialic Acid-Carrying Glycoproteins in Chinese Hamster Ovary Cells
• Two Polysialic Acid Synthases, Mouse ST8Sia II and IV, Synthesize Different Degrees of Polysialic Acids on Different Substrate Glycoproteins in Mouse Neuroblastoma Neuro2a Cells
• Molecular Cloning and Functional Analysis of Sialytransferases
• An immunohistochemical study of β1,4-galactosyltransferase in human skin tissue
• 糖転移酵素遺伝子の進化史
• 胃に生じる腸生に出現してくるルイスa抗原はルイス酵素の発現上昇によるものである
• Molecular Cloning and Genomic Analysis of Mouse GalNAc .ALPHA.2,6-Sialyltransferase (ST6GalNAc I).

もっと見る 閉じる

スポンサーリンク